3,863 research outputs found

    In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.

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    Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 µM 2,4-dichlorophenoxy acetic acid in combination with 2.2 µM benzylaminopurine. Shoots rooted readily on N6 basal medium supplemented with 1 g l-1 activated charcoal and 2.6 µM naphtalenacetic acid. The rooted shoots achieved 100% survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism

    Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers

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    Tigridia pavonia (L.f.) DC. is one of the important phytogenetic resources of México. This species is used as ornamental, food and medicinal purposes. Despite its ornamental and economic potential, there is little information about the genetic variability. In this study, randomly amplified polymorphic DNA (RAPD) primers of 10, 15 and 20 bases were used to assess the level of genetic variation among nine botanical varieties of Tigridia pavoniacollected in three localities within State of México. The total number fragments, polymorphic fragments, percentage of polymorphism and resolving power were greater for 15 base (55, 52, 94.5 and 5, respectively) and 20 base (47, 45, 95.7 and 3.8, respectively), in comparison with those obtained from 10 base primers (44, 41, 93.1 and 3.6, respectively).Resultsshowed the major effectiveness of 15 and 20 bases RAPD primers in the genetic differentiation of varieties as compared to 10 bases RAPD primers. The dendrograms based on un-weighted pair group method arithmetic average (UPGMA) analysis of the 10, 15, 20 and the pooled (10, 15 and 20) bases RAPD data were consistent in the clustering varieties, grouping them in two main clusters

    Modelización del costo de las empresas de inserción para la administración pública

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    This paper analyses a modern-day problem: the cost to government of jobs in social integration enterprises. At present there is a debate concerning their positive (subsidy-related) and negative aspects (regarding the savings they generate in social post-programmes and the revenue generated mainly by taxes and social security contributions). The study begins with an overview of social-integration enterprises, their possible development and the implication this has on the demand for public resources. In a second phase, the problem is analysed in greater detail and a possible approach is put forward based on a cost-profit analysis. The study then analyses previous research work and identifies the variables involved. Finally, a model enabling the cost analysis of the possible values of these variables is presented and hypothetical-predictive conclusions put forward.Social integration companies, cost-profit analysis, the solidarity-based economy, the social economy.

    Calidad de servicio y satisfacción del cliente en la empresa Mecánica Automotriz Salazar, Trujillo 2022

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    La presente investigación tuvo como objetivo determinar la relación que existe entre la calidad de servicio y la satisfacción del cliente de la Empresa Mecánica Automotriz Salazar, Trujillo 2022, la metodología que se utilizo fue de enfoque cuantitativo, diseño no experimental, corte transversal y nivel correlacional. Como población se tuvo una población infinita y se utilizó el muestreo probabilístico aleatorio simple siendo la muestra de 196 clientes, se utilizó como técnica la encuesta y como instrumentos al cuestionario. Se obtuvo como resultado que existe relación positiva alta estadísticamente con un Rho de Spearman de 0.896, cual muestra una correlación positiva muy alto, entre las variables calidad del servicio y satisfacción del cliente en la Empresa Mecánica Automotriz Salazar, Trujillo 2022 y un nivel de significancia menor a 0.005 Se concluyó la existencia de una relación entre la calidad de servicio y la satisfacción del cliente de la Empresa Mecánica Automotriz Salazar, Trujillo, 2022 quiere decir más eficiencia en la calidad de servicio mejor será la satisfacción de los clientes en la organización

    “Daily resilience”: sustainable strategies for urban fringe in three medium-sized inner Spanish cities

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    Urban resilience has evolved in the last time into a wider scope, related not only to facing major challenges or risks but to managing the complexity of urban systems and their “metabolism”, looking for more sustainable strategies. Consequently, it can be a very useful concept for both big and smaller cities, which are facing everyday urban life challenges. In a global context in which the urban phenomenon continues to spread, this paper aims to set a concept of “low regime”, “low intensity” or “daily” resilience as a specific and essential strategy for medium-sized cities in order to fulfil their role in the global concert of cities, through the historical analysis along the last five decades of three medium-sized inner Spanish cities: Vitoria, Zaragoza and Valladolid.These three cities, the most populated and complex among the medium-sized inner Spanish cities, are also capitals of their respective regions (the Basque Country, Aragon and Castile and León), and they represent the transition from an expansive model, in particular in their respective urban fringe, to the “urban resilience agenda”. In the sixties and seventies, they experienced an impressive growth, both physical and demographic. Later, in the eighties and nineties, the moderation in the population growth rates did not also moderate their physical expansion, which moved to the surroundings of the cities, until this growth came to an abrupt end in 2007 due to the economic crisis. Coming from a quite similar and homogenous cultural and urban context, Vitoria, Zaragoza and Valladolid have been developing undifferentiated peripheries, disrespectful with the conditions of the territory, until they started to adopt tailored strategies so as to guarantee a sustainable growth in the future and avoid eventual menaces for their urban territory.These new strategies aim to ensure the resilience of their respective urban fringe, because this is where the urban development tensions concentrate, and consist of composing an own profile of action, learning from the history of the city itself and from the natural values of their surrounding territory, and also keeping in mind the scarcity of economic resources in these medium-sized cities compared to bigger ones. Step by step, the singular history of each city, its “genius loci”, emerges as a solid foundation for these new strategies.This paper concludes that this idea of own identity seems to be resilient, and reveals that it can express itself through different tools (green infrastructure, urban regeneration and territorial planning) which in these three cities have found a useful topic to articulate a new integrated strategy for the “metabolism” of urban fringe in water systems: the Salburua wetlands and Zadorra river in Vitoria, protagonists of its greenbelt; the Ebro river in Zaragoza, recovered for the city after Expo 2008; and the network of rivers, canals and irrigation ditches in Valladolid, which stand as axes of a better relationship with the territory. All of them represent low cost smart and natural strategies that emerge as replicable examples of what “daily resilience” means and offers to these kind of medium-sized cities

    Comparative study of the discriminating capacity of dna markers and their effectiveness in establishing genetic relationships in the genus tigridia

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    Tigridia Jussieu is an endemic genus to Mexico and taxonomically difficult with limited information about its genetic variability. A diversity assessment conducted using different DNA markers as an inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) markers will be helpful in the establishment of a broad- based description for improved germplasm curation and the identification of germplasm for genome mapping and breeding of these species. Thus, the objective of this study was to characterize 15 wild species of Tigridia by using RAPD and ISSR molecular markers. This study was carried out in the laboratory of Plant Molecular Biology at the Faculty of Agricultural Sciences of the Universidad Autónoma del Estado de México between August and November of 2011. In this assay, 13 RAPD primers of 10, 15 and 20 b, and five ISSR primers of the anchored type (ASSR) of 17 b were used to assess the level of genetic variation among 15 wild species of Tigridia . With both markers there were 163 amplified bands of which 150 (92.02 %) were polymorphic. The RAPD primers of 10 b generated 12 specific bands with a polymorphism of 95.12 %, for 15 b primers those values were five and 82.93 %, and for 20 b primers eight and 94.59 %, respectively. The RAPD pooled primers presented a polymorphism of 90.76 %, the genetic distance (G D ) among the species ranged from 0.16 (between T. illecebrosa and T. huajuapanensis ) to 0.57 (between T. multiflora and T. augusta ). The ISSR primers showed more polymorphism(95.45 %) than RAPD primers. With ASSR primers the highest genetic association (G D = 0.89) was observed between T. mexicana ssp. mexicana and T. durangense , whereas the least related were T. vanhouttei spp. vanhouttei and T. multiflora (G D = 0.14). This study shows that 10 base random primers and 17 base anchored primers were more efficient to detect polymorphism and genetic differentiation among Tigridia species

    Planificación Integrada de Tiempos (PTI). Mejora de la productividad de trabajadores del conocimiento

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    Algunos estudios apuntan que el modelo de conexión permanente que traen las TIC dispersa la atención, disminuye la productividad y aumenta el estrés. Esta investigación presenta casos de empresas en los que se estudia la productividad de trabajadores del conocimiento

    Deterioro cognitivo postquirúrgico en pacientes con enfermedad coronaria y/o valvulopatía aórtica

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    Se muestra la presencia de deterioro cognitivo postquirúrgico en pacientes con enfermedad coronaria y/o valvulopatía aórticaUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

    Are circles isoperimetric in the plane with density er?

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    We prove that an isoperimetric region in R2 with density er must be convex and contain the origin, and provide numerical evidence that circles about the origin are isoperimetric, as predicted by the Log-Convex Density Conjecture
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